FIMM

P.O. Box 20
FI-00014
University of Helsinki
Finland

Contacts

2012 © Institute for Molecular Medicine Finland FIMM

ILLUMINA GENOME ANALYZER IIx

The Illumina Genome Analyzer IIx is a massively parallel sequencing platform for analyzing nucleic acid libraries in gigabase level. The GAIIx system produces over 50 Gb (320 M reads) of sequence in a run. The clonal bridge amplification within the physically enclosed flow cell allows quantitative applications such as gene expression profiling. An additional dimension for the Illumina GAII platform is introduced by the paired-end module which enables us to generate multiple sequence reads per flow cell. Paired reads are essential for detecting structural variants (paired-end & mate pair reads) and for multiplexing samples (indexing tags).

Applications and Services

FIMM Technology Center is ready to serve you in with the following GA II applications:

  1. Genome Sequencing
    • Paired-End DNA sequencing
    • Indexing (multiplexed DNA libraries)
  2. Exome sequencing
  3. Custom targeted re-sequencing
  4. Transcriptome analysis
    • smallRNA analysis (miRNA profiling)
    • mRNA Sequencing (RNA-Seq)
  5. Gene Regulation & Control Analysis
    • Chromatin Immunoprecipitation (ChIP-Seq)

The GAII workflow consists of four major steps:

solexa_workflow_2pstaa.jpg

FIMM Technology Center is ready to help you in all or selected steps of the workflow. Detailed technology overview at illumina.com

Supporting equipment for GAII applications in FIMM:

Contact person

Sequencing Lab

Pekka Ellonen

+358 50 415 4481

Pirkko Mattila

+358 50 317 5434