2009 © Institute for Molecular Medicine Finland FIMM
ILLUMINA GENOME ANALYZER II
The Genome Analyzer II is a massively parallel sequencing platform for analyzing nucleic acid libraries in gigabase level. The GAII system produces over 1.5 gigabases of sequence in a single run. The clonal bridge amplification within the physically enclosed flow cell allows quantitative applications such as gene expression profiling. An additional dimension for the GAII platform is introduced by the paired-end module which enables us to generate multiple sequence reads per flow cell. Paired reads are essential for detecting structural variants (paired-end & mate pair reads) and for multiplexing samples (indexing tags).
Applications and Services
FIMM Technology Center is ready to serve you in with the following GA II applications:
- Genome Sequencing
- Single-Ended DNA sequencing
- Paired-End DNA sequencing
- Indexing (multiplexed DNA libraries)
- Transcriptome analysis
- smallRNA analysis (miRNA profiling)
- Gene expression tag profiling
- mRNA Sequencing (RNA-SEQ)
- Gene Regulation & Control Analysis
- Chromatin Immunoprecipitation (ChIP-Seq)
and Expanded Sequencing Applications :
- Paired-end and Mate Pair Sequencing
- Multiplexed Sample Sequencing
The GAII workflow consists of four major steps:
FIMM Technology Center is ready to help you in all or selected steps of the workflow. Detailed technology overview at illumina.com
Supporting equipment for GAII applications in FIMM:
- Covaris S-2 for DNA shearing / RNA extraction
- Savant DNA-120 SpeedVac for concentrating DNA / RNA samples
- Agilent 2100 Bioanalyzer for analyzing DNA and RNA libraries
- NanoDrop ND-1000 for rapid DNA / RNA quantitation
- Illumina GAII computing cluster of over 30 processors and more than 40 terabytes of storage capacity.