Keynote Speaker Oliver Stegle
Oliver Stegle is a group leader at the EMBL European Bioinformatics Institute (EMBL-EBI) in Cambridge, UK. His group is developing advanced statistical approaches for analysing high-dimensional molecular traits in the context of genetic variation. He received his Ph.D. from the University of Cambridge, UK, in Physics in 2009, followed by a period as postdoctoral researcher at the Max Planck Campus in Tübingen, Germany. He moved to EMBL-EBI in November 2012 to establish his own research group. The team at EBI develops statistical approaches to link genetic and non-genetic factors to molecular variation. Most recent interests include the use of iPS cell models and single-cell technologies to elucidate molecular variations in the context of human disease.
Abstract of the talk
iPS cell models provide a unique opportunity for studying the consequences of genetic changes in disease relevant contexts. In this talk, I will discuss recent advances of using large panels of human induced pluripotent stem cells (iPSCs). The UK Human Induced Pluripotent Stem cell Initiative has generate and make available iPSC lines from 500 healthy donors (www.hipsci.org), along with comprehensive genomic data from these lines. As iPSC lines are increasingly being used as patient-specific disease models it is crucial to understand the sources of biological variability and heterogeneity in these lines. We describe a first map or regulatory variations in iPS cells and differentiated cell types, combining multi-omics high-throughput profiling using RNA-seq, proteomics profiling and single-cell readouts in differentiating cells.