Location

Genome-wide methylation analysis

Genome-wide methylation profiling is available at our genomics facility with Illumina Infinium MethylationEPIC array capable of analyzing over 850 000 methylation sites per sample at single-nucleotide resolution.

Pricing

Examples of analysis prices per sample:

Array

University of Helsinki

(includes 0% VAT)

Other academic

€ (includes 24% VAT)

16 samples

520.75 €

678.02

32

488.07 €

635.47 €

96

355.97 €

463.47 €

 

Notice that the arrays are for specific amount of samples and thus the number of samples in a project should be dividable with the amount of samples that can be analyzed on one array / obtainable in array kits, otherwise also empty slots or unused arrays shall be invoiced.

For more information about arrays or if you are a non-academic user please contact Päivi Lahermo or Kati Donner. A meeting can be organized, where all project details can be discussed.

 

Service description

The laboratory work is done by dedicated personnel with nearly 12 years of experience in Illumina analysis methods. Our laboratory uses an electronic lab note book (ELN) for documentation of the laboratory workflow and an in-house made LIMS system for management of genotype and sample data.

Projects are managed and quality controlled using Illumina GenomeStudio software by experienced specialists. Further analysis and release formats can be discussed with projects. A report of QC and analysis is included in data release.

All received and produced data is stored in a database at FIMM. FIMM guarantees the storage of your data for 5 years.

 

How to get started

If you are interested in methylation analysis with Illumina Epic array please contact Päivi Lahermo or Kati Donner. A meeting can be organized, where all project details can be discussed.

Customer provides

1) Documentation

A FIMM Service Agreement must be signed before the project can start in our laboratory. If your project is required by law to have an ethical permit you need to supply us with a copy of it.

 

2) Sample information

Provide the sample information using FIMM basic information form. Note! You need to enable the included macros. The filename should be FIMM_Perustietojen_luovutus_v2010.xlsm for the macros to work.

Fill up this sample submission form, print it and bring or send it to the lab together with the samples. Please also remember to submit the form electronically!

The gender information requested for in the FIMM basic information form is optional but central for detection of possible sample mix-ups in our quality control.

 

3) DNA samples

For the analysis we need good quality DNA in a concentration of 50 ng/ul in a minimum volume of 20-30 ul. Illumina recommends that the samples are diluted in 1 x TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).

Note! We recommend that the concentration is measured with two independent methods, e.g. in addition to NanoDrop spectrophotometer with PicoGreen or Qubit fluorometric quantification. If you use only one method it should be PicoGreen, Qubit dsDNA assay or other similar method.

For quality control purposes we recommend that there should be duplicated samples and that the groups deliver a suitable pair of methylated and non-methylated samples as controls for analysis with the project samples. In case control studies the case and control samples should be evenly distributed / mixed on the plates.

 

DNA shipping instructions

Ship the DNA samples on 96-well plates.

Seal the plates securely using either an adhesive seal, strip caps or a removable heat-sealed lid. Use Adhesive PCR foil (e.g Cat. # AB-0626, Thermo Fisher Scientific Abgene) only for shipments at room temperature. Make sure to attach the foil seal properly to each well by rubbing the surface with soft cloth or lab wipe. Make sure to protect the seals from punches. If you stack multiple plates, put cardboard or wadding layer between the plates.

Note! If you send the plates on dry ice an adhesive foil seal should not be used. Use removable heat-seal or strip caps instead!

Whenever possible, please avoid shipping on Thursdays and Fridays to avoid the samples arriving over the weekend. Pack the samples so that the plates or seals are not damaged during shipment. If you stack multiple plates put cardboard or wadding layer between the plates.

Please ask us for instructions before sending the samples via air courier!

Plate naming:

Mark the plate name (the same name as in the FIMM basic information form), sample concentration and date information clearly on the front side of the plate (see the image below for an example).

Frozen samples:

Frozen plates should be placed in the shipping container with enough dry ice/blocks to keep the samples frozen during shipment and any delays that may occur with the courier.

Shipping address:

FIMM Genotyping Lab
FIMM Technology Centre
Room: F215a
Biomedicum 2U
Tukholmankatu 8
00290 HELSINKI
FINLAND

 

Last updated: 14.05.2018 - 14:52