Samples for Cell line authentication can be sent in tubes. Provide all the other samples in skirted 96-well plates.
- 0,2 ml plate (Thermo Scientific Cat. # AB-0800_L or 4Titude Cat.# 4ti-0740)
- 0,8 ml plate (Thermo Scientific Cat. # AB-0765)
All samples within a project should be diluted in the same concentration. Note! We recommend that the concentration is measured with two independent methods, e.g. in addition to NanoDrop with PicoGreen or Qubit.
|Method||Sample requirement||Sample volume||Concentration||Diluted in|
|Sequenom||120 ng for first multiplex, 20 ng more for each additional multiplex||
12 µl / one multiplex + 2 µl for each additional multiplex
|10 ng/µl||Nuclease-free water|
|Roche LightCycler||120 ng for first SNP, 20 ng more for each additional SNP||12 µl + 2 ul for each additional SNP||10 ng/µl||Nuclease-free water|
|Epityper DNA Methylation Analysis||1000 ng||20 µl||50 ng/µl||Nuclease-free water|
|Illumina Arrays||500 ng||10 µl||50 ng/µl||1 x TE *|
|Illumina DNA Methylation Analysis||1000 - 1500 ng||20 - 30 µl||50 ng/µl||1 x TE|
|Cell Line Authentication||30 ng||6 µl||5 ng/µl||Nuclease-free water|
|Mouse Speed congenics||min. 250ng||10 µl||50 ng/µl||1 x TE|
*1 x TE (10 mM Tris-HCl, 1mM EDTA)
- Sequenom and Roche LightCycler: Provide 90 unique samples and two duplicate samples per 96-plate. The rest four wells are filled with controls by us.
- Illumina arrays: In case / control projects you should consider duplicating one or more samples per 96-well plate for quality control.