Location

Libraries

The HTB maintains a chemical collection of about 140 000 drugs and drug-like compounds that are ready to use for screening. For siRNA screens, the HTB has the Ambion Silencer® Select Human siRNA Library V4.

Since FIMM/HTB is a partner in the DDCB network, a national infrastructure for drug discovery and chemical biology research in Finland, it also distributes aliquots of compounds to academic research groups in Finland for the price of handling. Aliquots of the compounds are given every second Wednesday, please e-mail laura.turunen(a)fimm.fi and maria.nurmi(a)fimm.fi and elina.z.lehtinen(a)fimm.fi for inquiries. Instructions for HTB services.

Aliquots are given every second Wednesday: 4.1.2017, 18.1.2017, 1.2.2017, 15.2.2017, 1.3.2017, 15.3.2017, 29.3.2017, 12.4.2017, 26.4.2017, 10.5.2017, 24.5.2017, 7.6.2017, 21.6.2017.

Equipment

Labcyte Access robotic system

This automated setup is used for creating assay plates with chemicals and/or siRNAs.

  • Labcyte Echo 550 -acoustic dispenser (2.5 nl droplet)
  • Labcyte Echo 525 -acoustic dispenser (25 nl droplet)
  • Nexus XPeel -plate peeler
  • Agilent Plateloc -plate sealer
  • Agilent V-spin -centrifuge
  • Labcyte Deerac LX -bulkfiller (4 liquid channels)
  • Thermo Scientific Cytomat 10 -plate hotel

BeckmanCoulter integrated robotic system

This automated setup is used for splitting libraries on Labcyte ECHO source plates by pipetting, and/or for running fully automated screens with cell or biochemistry based assays including siRNA and/or chemicals.

  • Yaskawa Motoman Robotics Motoman -robotic arm to move plates in the system
  • Beckman Coulter Biomek FXP -pipetting robot
  • Molecular Devices Paradigm -plate reader
  • Thermo Scientific Multidrop Combi nl
  • Thermo Scientific Multidrop Combi (2 devices)
  • Thermo Scientific Cytomat 10 -plate incubator for cells
  • Nexus XPeel -plate peeler
  • Agilent Plateloc -plate sealer
  • Agilent V-spin -centrifuge
  • Delidding stations and plate hotels on robot deck

Microscopes and Plate readers

  • BMG Pherastar FS -plate reader (two devices)
  • BioTek Cytation 5 -plate reader and microscope

Other equipment

  • The Aushon Biosystems 2470 -Arrayer
  • BioTek EL406 -plate washer/dispenser
  • Thermo Scientific Multidrop Combi -dispenser (*3)
  • Roylan developments StoragePod chemical storage system
  • Agilent bar code labeler
  • Roche Lightcycler 480 II
  • Other small equipment (plate shaker, cell incubators, centrifuges, etc)
Last updated: 27.06.2017 - 12:44