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Next Generation Sequencing

Our NGS service portfolio covers the full sequencing process from study design to primary sequence analysis. We have developed  NGS workflows for a large variety of experiments for both DNA and RNA samples. Cost effective sequencing experiments are enabled through libraries with powerful indexing scheme (molecular barcodes). Currently our dual-indexing protocol supports multiplexing up to 559 individual libraries in a single sequencing lane - and this can be expanded even further. High multiplexing levels enable cost efficient sequencing experiments. FIMM Bioinformatics team provides in-depth analyses for your needs. A selection of NGS services is listed below:

Library preparation

  • Genomic DNA library preparation (single or dual indexing, paired-end)
  • RNA library preparation (transcriptomes, small RNA, indexed, paired-end)
  • Amplicon librariy preparation (single or dual indexing, paired-end)

Target enrichment

  • Exome enrichment (multiple methods)
  • Custom target enrichment (multiple methods)
  • Disease specific panels (custom or commercial)
  • mitochondrial DNA enrichment (rolling circle amplification or PCR)
  • Custom PCR Amplicon setup for ultra deep-sequencing (dual indexing, paired-end)

Sequencing

  • HiSeq HT run mode: up to 2x100nt paired ens reads
  • HiSeq Rapid run mode : up to 2x150nt paired ens reads
  • MiSeq runs:  up to 250nt paired-end reads
  • More information here and from laboratory staff

Primary sequence analysis

  • Together with the bioinformatics team we have developed following NGS analysis pipelines to facilitate your research:
  • VCP: Variant Calling Pipeline for re-seqeuncing experiments.
  • RNA-seq pipeline: transcriptome analysis
  • Amplicon pipeline: for analyzing ultra-deep sequencing experiments

How to get started?

  • Contact us by e-mail or phone
  • Get a FIMM account (note: you need to accept security certificate)
  • How to get started -guide to FIMM IT-services

Mailing list

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Last updated: 12.09.2017 - 15:54